Síguenos...

Biblioteca Vehice

Journal of Aquatic Animal Health

Case Report: Strawberry Disease in Farmed Chilean Rainbow Trout

Case Report: Strawberry Disease in Farmed Chilean Rainbow Trout
Carlos Sandoval, Jorge Infante, Jessica Abad, Hugh W. Ferguson, Enrique Paredes, Samuel Valdebenito, Alejandro J. Yáñez, Pedro Ilardi & Ruben Avendaño-Herrera.

To cite this article: Carlos Sandoval, Jorge Infante, Jessica Abad, Hugh W. Ferguson, Enrique Paredes, Samuel Valdebenito, Alejandro J. Yáñez, Pedro Ilardi & Ruben Avendaño-Herrera (2016) Case Report: Strawberry Disease in Farmed Chilean Rainbow Trout, Journal of Aquatic Animal Health, 28:1, 1-10, DOI: 10.1080/08997659.2015.1114534

Abstract
Strawberry disease is a chronic, nonlethal skin condition that affects Rainbow Trout Oncorhynchus mykiss in the United States and several European countries, where it is also known as red-mark syndrome. We provide the first identification and characterization of three strawberry disease outbreaks occurring at two aquaculture farms in southern Chile. Clinically affected fish weighing an average of 400 g presented multiple bright-red, usually raised, skin lesions on the flank, ventral surface, and dorsal surface. A PCR using Rickettsia-like-organism (RLO)-specific primers was performed on nine affected fish, and all skin samples were positive for the RLO 16S ribosomal RNA sequence. All PCR results for Flavobacterium psychrophilum and other bacterial and viral pathogens were negative. Histopathological examination of the skin lesions revealed extensive dermatitis, with severe lymphocytic infiltration in advanced cases. This report is the first to describe strawberry disease in farmed Chilean Rainbow Trout. Additional studies are needed to evaluate the risk for Rainbow Trout culture; fish challenge experiments should be performed to fulfill Koch’s postulates and to demonstrate that RLO is the cause of this disease.
Received December 27, 2014; accepted October 23, 2015.

Descarga Articulo

Transboundary and Emerging Diseases

Isolation, Characterization and Virulence Potential of Tenacibaculum dicentrarchi in Salmonid Cultures in Chile

R. Avendaño-Herrera1,2,3,*, R. Irgang1,2, C. Sandoval4, P. Moreno-Lira4, A. Houel5, E. Duchaud5, M. Poblete-Morales1,2, P. Nicolas6 and P. Ilardi4,*

1 Laboratorio de Patologıade Organismos Acuaticosy Biotecnologıa Acuıcola, Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andres Bello, Vin~a del Mar, Chile.
2 Interdisciplinary Center for Aquaculture Research (INCAR), Concepcion, Chile.
3 Centro de Investigacion Marina Quintay (CIMARQ), Quintay, Chile.
4 Research and Development Laboratory, Veterquımica S.A.,Santiago, Chile.
5 INRA, Virologie et Immunologie, Jouy-en-Josas, France
6 INRA, Mathematiques et Informatique Appliquees du Genome a l’Environnement, Jouy-en-Josas, France

Abstract
In this study, we isolated, identified and characterized isolates of Tenacibaculum dicentrarchi in Atlantic salmon (Salmo salar) farmed in Chile for the first time. In 2010 and 2014, mortalities were observed in Atlantic salmon (average weight 25–30 and 480–520 g, respectively) at an aquaculture centre in Puerto Montt, Chile. Severe tail rots, frayed fins and, in some cases, damaged gills were detected. Wet smear analyses of these lesions revealed a high occurrence of Gram-negative, filamentous bacteria. Microbiological analysis of infected gill and tail tissues yielded six bacterial isolates. All were identified as T. dicentrarchi through polyphasic taxonomy, which included phenotypic characterization, 16S rRNA sequencing and multilocus sequence typing. The latter method revealed a close relationship of the Chilean genotype with the T. dicentrarchi type strain and two Norwegian Atlantic cod (Gadus morhua) isolates. The pathogenic potential of the TdChD05 isolate was assessed by challenging Atlantic salmon and rainbow trout (Oncorhynchus mykiss) for one hour, which resulted in mean cumulative mortal- ity rates of 65% and 93%, respectively, as well as clinical signs 14 days post-chal- lenge. However, challenged Coho salmon (Oncorhynchus kisutch) presented no mortalities or clinical signs of infection. These findings indicate that the geo- graphical and host distribution of T. dicentrarchi is wider than previously estab- lished and that this bacterium may have negative impacts on salmonid cultures.

Descarga Articulo

Toxicología Acuática en Peces

Carlos Sandoval1,2, Enrique Paredes3, Manuel Ulloa2, Xavier Gutiérrez4, Gerardo Muñoz5, Claudio Arcos6
1 M.V., MSc (c). Escuela de Graduados, Fac. Ciencias Veterinarias, Universidad Austral de Chile.
2 Investigación & Desarrollo Laboratorio Veterquímica S.A.
3 M.V., Dr. med.vet. Instituto de Patología Animal, Universidad Austral de Chile.
4 Ing. Acui. MSc. Norwegian Institute for Water Research (NIVA).
5 M.V. Área Técnica Sealand Aquaculture.
6 M.V. Marine Harvest.

carlos.sandoval@histopathology.cl csandoval@veterquimica.cl eparedes@uach.cl mulloa@veterquimica.cl xavier.gutierrez@nivachile.cl gerardomunoz@sealand.cl claudio.arcos@marineharvest.com

Descarga Articulo

Inflamación del Músculo Esquelético y Cardíaco (HSMI)

Carlos Sandoval Hurtado; M.V. Escuela de Graduados, Fac. Ciencias Veterinarias, Universidad Austral de Chile. Investigación & Desarrollo
Biovac S.A. carlos.sandoval@biovac.cl Enrique Paredes Herbach; M.V., Dr. med.vet. Instituto de Patología Animal, Universidad Austral de Chile. eparedes@uach.cl
María José Navarrete Talloni; M.V., MPVM, PhD. Instituto de Patología Animal, Universidad Austral de Chile. mnavarrete@uach.cl

Descarga Articulo

Intoxicación por metales en peces

Carlos Sandoval Hurtado1,2, Enrique Paredes Herbach3, Manuel Ulloa Campos2

1M.V., MSc (c). Escuela de Graduados, Fac. Ciencias Veterinarias, Universidad Austral de Chile.
2Investigación & Desarrollo Laboratorio Veterquímica S.A.
3M.V., Dr. med.vet. Instituto de Patología Animal, Universidad Austral de Chile.
carlos.sandoval@histopathology.cl - csandoval@veterquimica.cl - eparedes@uach.cl - mulloa@veterquimica.cl www.patologia-veterinaria.com

Descarga Articulo

Amebiasis Branquial

Carlos Sandoval Hurtado1,2, Enrique Paredes Herbach3,

1 M.V., MSc (c). Escuela de Graduados, Fac. Ciencias Veterinarias, Universidad Austral de Chile.
2 Investigación & Desarrollo Laboratorio Veterquímica S.A.
3 M.V., Dr. med.vet. Instituto de Patología Animal, Universidad Austral de Chile.

Descarga Articulo

Exposición crónica al cobre

Carlos Sandoval Hurtado1, María Fernanda Molina12, Enrique Paredes Herbach3

1M.V., MSc (c). Escuela de Graduados, Fac. Ciencias Veterinarias, Universidad Austral de Chile. Vehice.
2Med. Veterinaria Universidad de Concepción. Vehice.
3M.V., Dr. Med. vet. Instituto de Patología Animal, Universidad Austral de Chile.

Descarga Articulo

Alteraciones en Etapa de Alevín de Saco Developmental Disorders in yolk - sac Fry

Carlos Sandoval Hurtado1,2, Enrique Paredes Herbach3, Manuel Ulloa Campos2

1M.V., MSc (c). Escuela de Graduados, Fac. Ciencias Veterinarias, Universidad Austral de Chile.
2Investigación & Desarrollo Laboratorio Biovac S.A.
3 M.V., Dr. med.vet. Instituto de Patología Animal, Universidad Austral de Chile.
carlos.sandoval@biovac.cl - carlos.sandoval@histopathology.cl eparedes@uach.cl - manuel.ulloa@biovac.cl www.patologia-veterinaria.com

Descarga Articulo

Patologías Branquiales en salmónidos

Carlos Sandoval Hurtado M.V. 
Laboratorio VeHiCe

carlos.sandoval@histopathology.cl - carlos.sandoval@biovac.cl
Enrique Paredes Herbach M.V., Dr. Med. Vet.

Universidad Austral de Chile, Instituto de Patología Veterinaria eparedes@uach.cl

Descarga Articulo

Enfermedades emergentes en salmónidos en la etapa de agua dulce

Carlos Sandoval Hurtado1,2, Enrique Paredes Herbach3, Manuel Ulloa Campos2 , Pedro Ilardi Lazcano2, Victor Valerio Yaeger4

1 M.V., MSc (c). Escuela de Graduados, Fac. Ciencias Veterinarias, Universidad Austral de Chile.
2 Laboratorio Investigación & Desarrollo Veterquímica S.A.
3 M.V., Dr. med.vet. Instituto de Patología Animal, Universidad Austral de Chile.
4 Ing. Acuicultura., 3 South Smart Solutions for engineering Ltda.

carlos.sandoval@histopathology.cl csandoval@veterquimica.cl, eparedes@uach.cl, mulloa@veterquimica. cl, pilardi@veterquimica.cl, vvalerio@3se.cl

Descarga Articulo

Síguenos...

logo

VETERINARY HISTOPATHOLOGY CENTER
Dir.: Libertad 590, Lintz, Puerto Montt, Región de Los Lagos, Chile, Teléfono: +56 65 271 0232